%0 Journal Article
%T Metabolic Flux Analysis of L-Arginine Fermentation in Corynebacterium glutamicum<br>L-精氨酸生物合成的代谢流量分析
%A ZHU Jin-Wei
%A CHEN Qing-Shan
%A ZHANG Wei-Guo
%A <br>朱进伟
%A 陈青山
%A 张伟国
%J 微生物学通报
%D 2008
%I 
%X In this paper, metabolic networks of the Corynebacterium glutamicum GWY020 and the two derivatives carrying additional mutations HUI821and GUI089 were established and modified. The concentrations of extra-cellular metabolites were determined under sub-steady-state (50 h~52 h) of the batch culture. The metabolic flux distribution maps of the three strains were obtained, compared and analyzed. These results indicate that the introduction of analog supersensitive marker or analog resistant marker skew the metabolic flux towards the formation of L-Arginine. This study revealed the usefulness of the metabolic flux analysis as a tool for verification of existing production strains. The analysis may play an important role in helping us to rationally re-design metabolism for further improvement of fermentation process.
%K L-Arginine
%K Corynebacterium glutamicum
%K Metabolic flux analysis<br>L-精氨酸
%K 谷氨酸棒杆菌
%K 代谢流量分析
%K 精氨酸
%K 生物合成
%K 代谢流量分析
%K Corynebacterium
%K glutamicum
%K Fermentation
%K Analysis
%K 手段
%K 设计育种
%K 导向
%K 突变
%K 敏感性
%K 结构类似物抗性
%K 分析角度
%K 方向
%K 节点
%K 变化
%K 发生
%K 流量分配
%K 结果
%K 影响
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=8B01866C93E2CA6720AA10837E0CBBC4&yid=67289AFF6305E306&vid=6209D9E8050195F5&iid=38B194292C032A66&sid=9DB5806568A196BC&eid=FF79159C7F60E5D3&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=10