%0 Journal Article
%T Cloning and Sequence Analysis of Aspartokinase Genes From Corynebacterium crenatum<br>钝齿棒杆菌天冬氨酸激酶基因的克隆和序列分析
%A Yangjian Liu
%A Yingzi Zhang
%A Jiang Wang
%A Yu Wang
%A Zhihua Yu
%A Jiuyuan Ding
%A <br>刘阳剑
%A 张英姿
%A 王绛
%A 王宇
%A 余志华
%A 丁久元
%J 微生物学报
%D 2002
%I 
%X Aspartokinase genes (ask) from wild-type Corynebacterium crenatum AS1.542 and an AEC-resistant mutant Corynebacterium crenatum CD945 were cloned and sequenced. Analysis of ask sequence shows a exchange in a single base pair at position 1199 from T to C, leading to an amino acid change in the beta subunit of Aspartokinase. Leu80 in the wild-type is converted to Pro80 in the feedback-resistant enzyme. The substitution is located in ACT domain, a region regulated by concentration of lysine. The ORF sequence of ask from C. crenatum AS 1.542 shows homologies of 97.23%, 97.55% and 97.62% to those from C. glutamicum, C. flavum and B. lactofermentum. And the amino acid sequence deduced from ORF displays homologies of 99.76%, 99.52% and 99.76%, respectively. But there is much variation in the upstream sequence of C. crenatum AS 1.542 ask promoter compared to those from other Corynebacteria.
%K Corynebacteria
%K Aspartokinase
%K Lysine
%K AEC resistance<br>棒杆菌，
%K 天冬氨酸激酶，
%K 赖氨酸，
%K AEC抗性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=A3F28B412E801718&yid=C3ACC247184A22C1&vid=ECE8E54D6034F642&iid=E158A972A605785F&sid=396DD691E964F390&eid=87545994EC2C1F12&journal_id=0001-6209&journal_name=微生物学报&referenced_num=1&reference_num=11