%0 Journal Article
%T Development of the 2148hk/rr Gene Knock-out Mutant of Streptococcus suis Type 2<br>Ⅱ型猪链球菌二元信号转导系统2148hk/rr基因敲除突变体的构建
%A CHENG Gong
%A LI Ming
%A ZHENG Feng
%A WANG Jing
%A WANG Chang-Jun
%A PAN Xiu-Zhen
%A FAN Hong-Jie
%A TANG Jia-Qi
%A <br>程 功
%A 李 明
%A 郑 峰
%A 王 晶
%A 王长军
%A 潘秀珍
%A 范红结
%A 唐家琪
%J 微生物学通报
%D 2008
%I 
%X To construct gene knock-out mutant of a two-component signal transduction system named 2148hk/rr in Streptococcus suis type 2 virulent strain 05ZYH33. Recombinant gene knock-out vector was constructed consisting of Spcr cassette with flanking homology regions to the target genes, the isogenic 2148hk/rr-deficient mutant was screened by allelic replacement. PCR analysis and Southern hybridization confirmed that the coding genes of 2148hk/rr were replaced completely by spcr cassette. Conclusion The mutant of 05ZYH33 2148hk/rr system was successfully constructed, which laid the foundation for farther research on the role of this two-component signal transduction system during infection.
%K Streptococcus suis type 2
%K Two-component signal transduction system
%K Gene knock-out
%K Mutant<br>猪链球菌2型
%K 二元信号转导系统
%K 基因敲除
%K 突变体
%K Ⅱ型猪链球菌
%K 信号转导系统
%K 基因敲除
%K 突变体构建
%K Development
%K Type
%K Streptococcus
%K Mutant
%K 作用
%K 致病过程
%K 调控系统
%K 基因替代
%K 抗性基因
%K 完全
%K 显示
%K 结果
%K 杂交
%K Southern
%K 分析
%K 编码基因
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=FC509B53E16FB7BF8BD79171326BBC71&yid=67289AFF6305E306&vid=6209D9E8050195F5&iid=CA4FD0336C81A37A&sid=C5154311167311FE&eid=771469D9D58C34FF&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=17