%0 Journal Article
%T CONSTRUCTION OF CYTOPLASMIC RANDOM PEPTIDE LIBRARY<br>胞内随机序列多肽库的构建
%A Li Chuanzhao Liu Yule Ye Yin Yang Wending Tian Po
%A <br>李传昭
%A 刘玉乐
%A 叶寅
%A 杨文定
%A 田波
%J 微生物学报
%D 1995
%I 
%X A nonapeptide library was constructed by cloning randomly synthesizedoligonucleotides into ptacG4. Millions of different nonapeptides can be expressed inE. coli cell in a loop of GAL4 zinc finger structure, which was derived from the plasmidptacG4. Compared with the surface displayed peptide library, cytoplasmic library can bescreened by biological activity and can be easily kept in expression plasmid.
%K Cytoplasmic random peptide library
%K Nonapeptide
%K Construction<br>Cytoplasmic
%K random
%K peptide
%K library
%K Nonapeptide
%K Construction
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=3C4F9590C2FB60F4F9A575B3FD4EBE0F&yid=BBCD5003575B2B5F&vid=6209D9E8050195F5&iid=38B194292C032A66&sid=CEC789B3C68C3BB3&eid=AD16A18DBD734D13&journal_id=0001-6209&journal_name=微生物学报&referenced_num=1&reference_num=1