%0 Journal Article
%T Construction and Characterization of a ppbR gene Mutant of Pseudomonas sp. M18<br>假单胞菌M18调控基因ppbR的克隆及功能研究
%A ZHENG Fei
%A HUANG Xian-Qing
%A XU Yu-Quan
%A ZHANG Xue-Hong
%A <br>郑 斐
%A 黄显清
%A 许煜泉
%A 张雪洪
%J 微生物学通报
%D 2008
%I 
%X Pseudomonas sp. M18, one of plant-growth-promoting rhizobacteria, can produce secondary metabolites including phenazine-1-carboxylic acid (PCA) and pyoluteorin (Plt). PA2572 gene coding protein is a probable two-component response regulator in Pseudomonas according to homologous speculations. In order to investigate its genetic function, PA2572 homologous gene, ppbR, was amplified from M18 genome, inactivated by inserting a Gm cassette. The resulting reconstruct was introduced into the M18 genome using homologous recombination technique, so as to obtain the null mutant M18P. The results showed that the M18P has less flagellar swimming and swarming motility, and yielded fewer PCA. The production of PCA was only 50% of the wild type. However, there was no remarkable difference between mutant and wild type in producing pyoluteorin in KMB medium.
%K Pseudomonas sp
%K M18
%K ppbR
%K Two-component regulatory system
%K Swimming mobility
%K Swarming mobility
%K Phenazine-1-carboxic acid<br>假单胞菌M18
%K ppbR
%K 双元调控系统
%K 泳动能力
%K 群集运动能力
%K 吩嗪-1-羧酸
%K 假单胞菌
%K 调控基因
%K 克隆
%K 功能研究
%K Pseudomonas
%K Mutant
%K gene
%K Characterization
%K 差异
%K 培养基
%K 积累量
%K 发酵液
%K 野生型
%K 合成
%K 运动能力
%K 泳动
%K 结果
%K 突变株
%K 技术构建
%K 同源重组
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=802F72120C46CCD62E29D6553040AF69&yid=67289AFF6305E306&vid=6209D9E8050195F5&iid=0B39A22176CE99FB&sid=8E3E075781370D7B&eid=E440CD1BE660F9BE&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=11