%0 Journal Article
%T Construction of A Set of Secreting Expression Vectors for Saccharomyces cerevisiea<br>一种新型酿酒酵母附加型分泌表达载体的构建
%A Zhao Yingyi
%A Liang Shizhong
%A Huang Kun
%A Huang Ribo
%A <br>赵颖怡
%A 梁世中
%A 黄鲲
%A 黄日波
%J 微生物学报
%D 2002
%I 
%X The DNA fragment ecoding the Signal peptide of inulinase of Kluyveromyces smarxianu was synthesized chemically. This fragment was cloned in-frame in the expression vector pYES2 of Saccharomyces cerevisiae, resulting in a set of new secreting expression vectors pYES2 I, pYES2 II, pYES2 III. The L-Asparaginase gene (ASN) of E. coli and alpha-acetylactate decarboxylase gene (ALDC) of B. brevis which were amplified by PCR and cloned into the new vectors respectively were transformed into Saccharomyces cerevisia, and most of enzyme activities were secreted into the medium. The new secreting expression vectors still have excellent segregational stability even after growth for 100 h in the absence of selective pressure.
%K Saccharomyces cerevisiae
%K Signal peptide
%K Secreting expression vector<br>酿酒酵母，
%K 信号肽，
%K 分泌表达载体
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=918F85874BE75DBF&yid=C3ACC247184A22C1&vid=ECE8E54D6034F642&iid=E158A972A605785F&sid=6D6BFCF0101BC091&eid=8D75AD3BD0D1BCC5&journal_id=0001-6209&journal_name=微生物学报&referenced_num=1&reference_num=10