%0 Journal Article
%T Study on Effeet of Vitreoscilla Hemoglobin Gene Expression on Growth and Metabolism of Streptomyces aureofaciens<br>透明颤菌血红蛋白基因表达对金色链霉菌生长代谢的影响
%A Meng Chun
%A Ye Qin
%A Qiu Li
%A Shi Xian'ai
%A Su Wenjin
%A Guo Yanghao
%A <br>孟春
%A 叶勤
%A 邱荔
%A 石贤爱
%A 苏文金
%A 郭养浩
%J 微生物学报
%D 2002
%I 
%X Vitreoscilla hemoglobin gene was expressed in S. aureofaciens through promoter of tetracycline resistance gene. Characteristics of S. aureofaciens growth and metabolism were studied in 1 m3 fermenter. In high dissolved oxygen concentrations, expression of hemoglobin gene had little effect on growth and metabolism of S. aureofaciens, and there were no obvious differences between engineering strain and control. The chlortetracycline of engineering strain was 22905 u/mL and of control was 22896 u/mL respectively. Under conditions of low dissolved oxygen, expression of hemoglobin enhanced growth, maintenance of energetic mycelium configuration and chlortetracycline yield of S. aureofaciens: the mycelium concentrations of engineering strain were more about 5%-10% and yield was more 11.4% than control.
%K Vitreoscilla
%K hemoglobin Gene
%K Streptomyces aureofaciens
%K Dissolved oxygen concentration
%K Chlortetracycline<br>透明颤菌血红蛋白基因，金色链霉菌，溶解氧浓度，金霉素
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=FB95A814C682E5F3&yid=C3ACC247184A22C1&vid=ECE8E54D6034F642&iid=E158A972A605785F&sid=6B3068A7C27BD349&eid=D1D63D047E37A053&journal_id=0001-6209&journal_name=微生物学报&referenced_num=3&reference_num=9