%0 Journal Article
%T Proteomic analysis of effects of iron depletion on Streptococcus pyogenes MGAS5005<br>应用蛋白质组学的方法分析缺铁培养对化脓性链球菌MGAS5005的影响
%A 吕洁
%A 余光创
%A 孙正华
%A 王南杰
%A 朱颖
%A 王红翠
%A 孙雪松
%J 微生物学通报
%D 2012
%I 
%X Objective] Analyze the effects of iron deficiency on Streptococcus pyogenes, and find the key proteins in the iron acquisition systems. Methods] Streptococcus pyogenes was cultured in THY medium with or without iron. Total proteins were extracted for the 2DE-gel analysis. The altered proteins upon iron depletion were identified by mass spectrometry. Further bioinformatics analyzed the relationship between key differentially expressed proteins. Results] The 20 alterated protein were identified. With Cytoscape analysis of the betweenness of these proteins, 5 bottleneck proteins were detected. Conclusion] The results showed that iron depletion affect the cellular biosynthetic processes, the metabolic process of compound containing nitrogen and the macromolecular metabolic process etc. This study laid a foundation for further studying the mechanisms of the iron metabolism in bacteria.
%K Streptococcus pyogenes
%K metalloprotein
%K iron
%K proteomics<br>化脓性链球菌
%K 金属蛋白
%K 铁
%K 蛋白质组学
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=EDE30CAACE5A91470A6959C3C900691B&yid=99E9153A83D4CB11&vid=7C3A4C1EE6A45749&iid=E158A972A605785F&sid=7ED2DDC775D689E1&eid=588D0ADCB4F3A920&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=25