%0 Journal Article
%T STUDIES ON PURIFICATION AND SOME PROPERTIES OF NISIN FROM LACTOCOCCUS LACTIS SUBSP. LACTIS AL2<br>乳酸乳酸球菌AL2产生的乳链菌肽的提纯和性质
%A Chen Xiuzhu He Song Long Lihong Huan Liandong Xue Yugu
%A <br>陈秀珠
%A 何松
%A 龙力红
%A 还连栋
%A 薛禹谷
%J 微生物学报
%D 1996
%I 
%X Nisin from Lactococcus lactis subsp. lactis AL2 was extracted with n-propanol from NaCl-saturated culture and purified by ion-exchange chromotography on CM-Sephadex C-25. Nisin was purified 1.63 fold with a yield of 41.7%. The molecular weight of nisin was determined by SDS-PAGE to be about 3500. Nisin activity was stable at low pH and sensitive to digestion by a-chymotrypsin. Nisin is capable of inhibiting a broad range of gram-positive bacteria. In contrast, the gram-negative bacteria, yeasts, molds and Nip L. lactis subsp. lactis ATCC11454 were not inhibited.
%K Lactococcus lactis subsp
%K lactis AL2
%K Nisin
%K Purification and properties<br>乳酸乳酸球菌AL2
%K 乳链菌肽
%K 提纯和性质
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=6D70FC6F56196D682DEB664ECAB6AC02&yid=8A15F8B0AA0E5323&vid=933658645952ED9F&iid=E158A972A605785F&sid=1A363081E1FF7014&eid=7979125BBE749348&journal_id=0001-6209&journal_name=微生物学报&referenced_num=12&reference_num=4