%0 Journal Article
%T Preliminary Studies on the Apoptosis of Spodoptera litura Cells Induced by TWO Baculoviruses<br>杆状病毒诱导昆虫细胞凋亡的初步研究
%A Yao Lunguang Du Changsheng Peng Jianxin Hong Huazhu
%A <br>姚伦广
%A 杜昌升
%A 彭建新
%A 洪华珠
%J 微生物学报
%D 2002
%I 
%X Microscopic examination of Spodoptera litura(SL-1) cell infected with AcMNPV or SfaMNPV revealed progressive cell blebbing starting at 8 h-12 h postinfection and culminating in total cell destruction at 24 h postinfection. The fragmentation of the infected cell nuclei was observed by stained with the specific fluorescent dye DAPI. Agarose gel electrophoresis analysis of the DNA extracted from infected cells show typical DNA ladder. All these supported that both viruses infected SL-1 cells undergo apoptosis. Through lipofectin transinfection, we observed that only the DNA of AcMNPV or SfaMNPV can also induce apopotosis of SL-1 cell. Virus titer analysis revealed that neither viruses can duplicate in SL-1 cells.
%K Baculovirus
%K Spodoptera litura cell
%K Apopotosis
%K DNA ladder<br>杆状病毒，
%K 斜纹夜蛾细胞系，
%K 细胞凋亡，
%K 梯形带
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=47C18FE9A4CC41EA&yid=C3ACC247184A22C1&vid=ECE8E54D6034F642&iid=38B194292C032A66&sid=A7AE820C12CC9AD3&eid=E46298901AD1B3E298DD4AAFCCBC7801&journal_id=0001-6209&journal_name=微生物学报&referenced_num=6&reference_num=5