%0 Journal Article
%T CLONING AND IDENTIFICATION OF fliC~i GENE OF SALMONELLA TYPHIMURIUM<br>鼠伤寒沙门氏菌Ⅰ相鞭毛蛋白基因fliC~I的克隆和鉴定
%A Cai Xuezhong Jiao Xinan Zhang Rukuan Liu Xiufan
%A <br>蔡学忠
%A 焦新安
%A 张如宽
%A 刘秀梵
%J 微生物学报
%D 1996
%I 
%X Purified Salmonella typhimurium 8705 chromosomal DNA was digested with EcoR I. DNA fragments above 400bp were obtained by Sepharcyl S-400 chromatography and cloned into vector pGEM-3Zf (-) , then transformed into host cell LC2a(hag-is recA-). One out of 6013 transformants was found to be motile and this clone was named pGI4015. Miniprep proved that pG I 4015 contained an inserted fragment about 15.3kb. Motility / inhibition tests as well as Southern blot hybridization showed that pG I 4015 bear the H-li gene of S. typhimurium. With the aid of BamH I and Sal I site, sequences unrelated to flagellin in the cloned DNA fragment was removed, and a 3.8kb fragment containing fliC gene was subcloned.
%K Salmonella typhimurium
%K fliC1 gene
%K Clone<br>鼠伤寒沙门氏菌
%K I相鞭毛蛋白基因fliC~I
%K 克隆
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=D9718EE2E5A1BC8A9FF12418EB2773BC&yid=8A15F8B0AA0E5323&vid=933658645952ED9F&iid=E158A972A605785F&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=0