%0 Journal Article
%T Two-stage pH control strategy of dextran dextrinase production by Gluconobacter oxydans DSM 2003<br>氧化葡萄糖杆菌合成右旋糖酐糊精酶的pH两阶段控制策略
%A WANG Shu
%A MAO Xiang-Zhao
%A LI Fu-Li
%A WEI Dong-Zhi
%A <br>王舒
%A 毛相朝
%A 李福利
%A 魏东芝
%J 微生物学通报
%D 2011
%I 
%X In order to enhance the yield of dextran dextrinase (DDase) produced by Gluconobacter oxydans DSM 2003, the effects of pH from 3.5 to 6 on cell growth and DDase activity were investigated in a 3 L fermentor. Based on time courses of cell growth and DDase activity, we developed a two-stage pH control strategy, in which pH was controlled at 5.0 for the first 6 h and then 4.0 for remaining time. Under the optimized strategy, the production of DDase had a significant improvement. Moreover, the maximal DDase activity reached 4.03 U/mL, 38.5% and 1 147% more than that from the strategy without pH control and in the 250 mL shake flasks, respectively. Meanwhile, the fermentation time of DDase in the fermentor was also shortened from 47 h to 15 h compared to that in 250 mL shake flasks.
%K Gluconobacter oxydans
%K Dextran dextrinase
%K Dextran
%K pH control strategy<br>氧化葡萄糖杆菌
%K 右旋糖酐糊精酶
%K 右旋糖酐
%K pH分段控制策略
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=20E65ECF19352841C516E13F263F5D34&yid=9377ED8094509821&vid=16D8618C6164A3ED&iid=5D311CA918CA9A03&sid=EF10DC5E94EFF05A&eid=545EC3172B3789BC&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=15