%0 Journal Article
%T Screening and identification of cold-active amylase strain C2 and its amylase characterization<br>低温淀粉酶菌株C2的分离鉴定及其产低温淀粉酶的酶学性质
%A FENG Xu-Ming
%A CHI Nai-Yu
%A ZHANG Qing-Fang
%A <br>冯旭明
%A 迟乃玉
%A 张庆芳
%J 微生物学通报
%D 2011
%I 
%X Strain producing cold-active amylase want to be isolated. Enzymatic properties of the strain will be determined. Screening strains from The Black mountant. Determine its species by its morphological, the physiological and biochemical properties and 16S rDNA sequence analysis, to study its enzymatic properties. A strain C2 producing cold-active amylase was identified as Exiguobacterium sp.. Enzymatic properties showed that the optimum pH value is 7.5 and the optimum reaction temperature is 25 °C, but the thermal stability of the enzyme is relatively poor. The amylase activity was activated by Ca2+ and Fe2+, and was inhibited by Cu2+, Ni2+ and Go2+. By the identification of TLC, the product of enzymolysis is glucose. It is indicates that the strain can product cold-active starch glucoamylase. Amylase produced by strain C2 in line with the nature of cold-active amylase, it deserves to be further researched.
%K Cold-active amylase
%K 16S rDNA
%K Exiguobacterium sp
%K Enzymatic properties<br>低温淀粉酶
%K 16S
%K rDNA
%K 微小杆菌
%K 酶学性质
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=8AC6258FD9B9B70106DDD0728600A8F6&yid=9377ED8094509821&vid=16D8618C6164A3ED&iid=59906B3B2830C2C5&sid=ED018FBF5E47A8BD&eid=1FDC6DA6F37D438C&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=16