%0 Journal Article
%T Biogenesis of iron sulfur cluster of Acidithiobacillus ferrooxidans<br>嗜酸氧化亚铁硫杆菌ISC铁硫簇系统的合成
%A QIAN Lin
%A ZHENG Chun-Li
%A LIU Jian-She
%A <br>钱林
%A 郑春丽
%A 柳建设
%J 微生物学通报
%D 2012
%I 
%X Objective] Iron sulfur cluster are one of the most ancient and ubiquitous redox centre in almost all living organism and play an important role in photosynthesis, respiration and nitrogen fixation. Methods] In this study, three key proteins (IscS, IscU, IscA) involved in iron sulfur cluster assembly from A. ferrooxidans ATCC 23270 was cloned and expressed in Escherichia coli. Results] IscS is a cysteine desulfurase, IscU is a scaffold protein, IscA has strong iron binding activity which acts an iron chaperon. Conclusion] The Fe-IscA can provide iron for the assembly of transient iron sulfur cluster in IscU in the presence of IscS and L-cysteine in vitro.
%K Acidithiobacillus ferrooxidans
%K Iron sulfur cluster
%K Biogenesis
%K IscA<br>嗜酸氧化亚铁硫杆菌
%K 铁硫簇
%K 生物合成
%K IscA
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=B5A93E035C82D3EF528F3C5EC995FD4E&yid=99E9153A83D4CB11&vid=7C3A4C1EE6A45749&iid=5D311CA918CA9A03&sid=DE3C727FE2D9509D&eid=8DEAC935CD342902&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=0