%0 Journal Article
%T Proteome Analysis of Secreted Proteins of Brucella melitensis<br>羊布鲁氏菌的分泌蛋白质组分析
%A WANG Yu-Fei
%A QU Qing
%A QIAO Feng
%A ZHONG Zhi-Jun
%A DU Xin-Ying
%A WANG Zhou-Ji
%A CHEN Ze-Liang
%A HUANG Liu-Yu
%A <br>王玉飞
%A 曲 勍
%A 乔 凤
%A 钟志军
%A 杜昕颖
%A 汪舟佳
%A 陈泽良
%A 黄留玉
%J 微生物学通报
%D 2009
%I 
%X Secreted proteins (the secretome) of the Brucella may mediate important pathogen-host interactions, but such proteins are technically difficult to analyze. In the present study, we describe an approach to identify Brucella melitensis secreted proteins by using proteome analysis. TCA-acetone was used to recovery extracellular proteins from the culture of Brucella melitensis, and two-dimensional gel electrophoresis followed by mass spectrometry for protein resolution and identification. At last, 40 proteins were identified. These proteins included substrate-binding proteins of ABC transporters, outermembrane proteins and heat shock proteins. The identification of these secreted proteins provides important clues for the understanding of the molecular mechanism of Brucella and interesting candidates for the vaccine to treat or prevent Brucella infections.
%K Brucella
%K Secreted protein
%K Two-dimension electrophoresis (2-DE)
%K Mass spectrometry<br>布鲁氏菌
%K 分泌蛋白
%K 双向电泳
%K 质谱
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=0DA4219074ADF88C98FB06C234D6C463&yid=DE12191FBD62783C&vid=933658645952ED9F&iid=5D311CA918CA9A03&sid=19E8BB8BE90732B2&eid=CD1AB74EAF0667FF&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=1&reference_num=10