%0 Journal Article
%T EXPRESSION AND ACTIVITY ANALYSIS OF THE HUMAN GLUTATHIONE-S-TRANSFERASE IN LACTOCOCCUS LACTIS<br>人谷胱甘肽硫转移酶A1在乳酸乳球菌中的表达及活性研究
%A Xiang Hua
%A Xhang Yiqing
%A Wei Wenzhong
%A Tan Huarong
%A <br>向华
%A 张亦清
%A 卫文仲
%A 谭华荣
%J 微生物学报
%D 2000
%I 
%X Theglutathione S transferaseA1cDNAwasamplifiedfromhumanlivertotal RNAsbyRT PCRandwasclonedintoaEscherichiacoliexpressionvectorpET2 3b ,then therecombinantplasmidpET2 3bhgstwasintroducedintoE .coliBL2 1 (DE3)andinduced byIPTG ,thehigh levelexpressionofhGSTA1appearedintheE .colicells.ThecDNAen codinghGSTA1wassubclonedintopMG36e,alactococcalexpressionvector,andintroduced intoLactococcuslactisMG1 36 3byelectroporation .Inthepositivetransformants,thehGS TA1wasexpressedasafusionproteinwhichwasverifiedbySDS PAGEandWesternblot. ThehGSTA1frombothE .coliandL .lactiswaspurifiedbyaffinitychromatographyon glutathione agaroseandallshowedenzymaticactivity .Thepotentialapplicationofthere combinantLactococcuslactisinfunctionalfoodwasalsodisscussed .
%K Glutathione-S-transferase
%K Gene cloning and expression
%K Lactococcus lactis<br>谷胱甘肽硫转移酶
%K 基因克隆表达
%K 乳酸乳球菌
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=66C88E0F31679251&yid=9806D0D4EAA9BED3&vid=1371F55DA51B6E64&iid=0B39A22176CE99FB&sid=7C72DBC13F2D71EC&eid=09E495F616948E78&journal_id=0001-6209&journal_name=微生物学报&referenced_num=10&reference_num=14