%0 Journal Article
%T CLONING AND EXPRESSION OF THE GENE ENCODING NOVEL a-AMYLASE FROM SULFOLOBUS SHIBATAE IN E. COLI<br>芝田硫化叶菌新型α-淀粉酶基因在大肠杆菌的克隆和表达
%A Liu Li
%A Chen Wei
%A Jin Cheng
%A <br>刘莉
%A 陈炜
%A 金城
%J 微生物学报
%D 2000
%I 
%X A novel α-amylase gene was amplified from Sulfolobus shibatae by using PCR technique.The amplified 1.7kb DNA fragment was inserted into an expression vector pBV220 to yield the recombinant plasmid pSBAM. The novel α-amylase gene in pSBAM was expressed in E. coli. The production of the novel α-amylase activity reached over 8 units/100mL of the culture. The molecular weight of this enzyme was about 61kD by SDS-PAGE. The expressed novel α-amylase protein in E.coli DHSα accounted for about 20 % of the total protein in the recombinant cell. The cooperative action of the novel α-amylase and the maltooligosyltrehalose synthase from Sulfolobus shibatae was investigated and trehalose was detected by using HPLC analysis when using amylose and partial starch hydrolysates as substrates.
%K 新型α淀粉酶，
%K 芝田硫化叶菌，
%K 大肠杆菌，
%K 基因的克隆和表达
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=82639B013A7AD6947FCFA7FB55F06F4E&yid=9806D0D4EAA9BED3&vid=1371F55DA51B6E64&iid=38B194292C032A66&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=0