%0 Journal Article
%T STUDIES ON FERMENTATION CONDITIONS OF CYTOKININ PRODUCED BY RHIZOBIA STRAIN 4012a<br>根瘤菌4012a菌株产细胞分裂素发酵条件的研究
%A Jia Xiaoming
%A Chen Shengming
%A Min Hang
%A Qian Zeshu
%A <br>贾小明
%A 陈声明
%A 闵航
%A 钱泽澍
%J 微生物学报
%D 1998
%I 
%X The conditions of cytokinin fermentation of the rhizobia strain 4012a were detected by the ELISA. The results indicated that the optimal medium for cytokinin production by strain 4012a was composed of glucose 10 g/L, (NH4)2SO4 1.0, K2HPO4.3H2O 0.6, MgSO4.7H2O 0.1, CaCl2.2H2O 0.4, FeCl3.6H2O 0.04, Na2MoO4.2H2O 0.1 mg/L, calcium pantothenate 100 micrograms/L, adenine 200 mg/L. When strain 4012a was grown in 250 ml flask containing 50 ml of the medium on the rotary shaker (150 r/min) at 27 degrees C for 96 h, the yield of CTK 908 micrograms/L culture solution was obtained. It displayed bioactivity kinetin equivalents (KE) 1 mg/L medium with the radish cotyledon expansion test.
%K Cytokinin
%K Rhizobla
%K Fermentation
%K ELISA<br>细胞分裂素
%K 根瘤菌
%K 发酵
%K 酶标免疫检测法（ELTSA）
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=B1CA673B9B2517D5D11F8303CAE6F9D1&yid=8CAA3A429E3EA654&vid=16D8618C6164A3ED&iid=38B194292C032A66&sid=D5C73DEF4CF8FAF3&eid=A1266CF37D675CF1&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=3