%0 Journal Article
%T CONSTRUCTION OF A NOVEL Bm NPV Bac to Bac SYSTEM<br>一种新型家蚕核多角体病毒Bac to Bac系统的构建
%A Deng Xiaozhao
%A Zhu Ying
%A Diao Zhenyu
%A Qi Yipeng
%A Zhou Zongan
%A <br>邓小昭
%A 朱应
%A 刁振宇
%A 齐义鹏
%A 周宗安
%J 微生物学报
%D 2000
%I 
%X A Bi-Shuttle vector Bm-Bacmid was constructed by co-infecting Bm N cells with wild type genomic DNA from BmNPV and Ac-Bacmid DNA. It could not only replicate in E. coli cells as a large plasmid and but also remain infectious when induced into Bm N or Sf9 cells. Recombinant virus rBmHBe was obtained after transposition of a donor plasmid carrying Hepatitis Be antigen gene (HBeAg) into att Tn7, and was demonstrated by Southern blotting. SDS-PAGE analysis showed that HBeAg gene were highly expressed in Bm N cells. By ELISA testing, the highest antigenecity titer of HBeAg protein in cell cultural medium was up to a dilution of 1:32,000. Although HBeAg protein also presented in the Bm N cells the titer was only 1:2000. The HBcAg protein was much fewer than HBeAg (< 1:160) whatever in culture medium and in cells. The results showed that Bm N cells was able to recognize the signal peptide sequence and cut it correctly for HBeAg protein's excreting production.
%K Bm NPV
%K Ac-Bacmid
%K Transposon TnT
%K HBeAg
%K Gene expression<br>BmNPV
%K Bac
%K to
%K Bac策略
%K 表达系统
%K HBcAg
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=E5310CF8EB7EDB0D&yid=9806D0D4EAA9BED3&vid=1371F55DA51B6E64&iid=0B39A22176CE99FB&sid=7CE3F1F20DE6B307&eid=1B97AE5098AEB49C&journal_id=0001-6209&journal_name=微生物学报&referenced_num=9&reference_num=11