%0 Journal Article
%T THE KEY ENZYMES OF METABOLISMS OF GLYCEROL IN CANDIDA GLYCEROLGENESIS<br>产甘油假丝酵母甘油代谢关键酶的研究
%A Wang Zhengxiang
%A Zhuge Jian
%A Cao Yu
%A Chen Jun
%A Fang Huiying
%A <br>王正祥
%A 诸葛健
%A 曹钰
%A 陈珺
%A 方慧英
%J 微生物学报
%D 2000
%I 
%X The specific enzymes for glycerol formation and dissimilation in Candida glycerolgenesis were studied. C.glycerolgenesis hardly assimilated glycerol without glucose and a little amount of glucose remarkably stimulated this dissimilation pathway and the activity and expression of mitochondrial glycerol 3 phosphate dehydrogenase was induced strongly by glycerol 3 phosphate and repressed by glucose metabolism. During glycerol production fermented by C.glycerolgenesis , the activity of cytoplasmic glycerol 3 phosphate dehydrogenase maintained at a higher level and existed two peaks of activity, which appeared at 36h and 60h, respectively. The activity level of the first activity peak determines glycerol productivity and becomes a key enzyme of glycerol formation. The activity of glycerol 3 phosphatase maintained at a higher level just between 18h and 48h and appeared on paek activity at 36h. In that period, the activity of glycerol 3 phosphatase was up to more than two times of cytoplasmic glycerol 3 phosphate dehydrogenase and that was inosculated with the phase of rapid accumulation of glycerol during glycerol production by C.glycerolgenesis . In the phase of slowly accumulation of glycerol between 48h and 72h, the activity of glycerol 3 phosphatase decreased below that of cytoplasmic glycerol 3 phosphate dehydrogenase. So, cytoplasmic glycerol 3 phosphate dehydrogenase is a key enzyme of glycerol formation in the phase of rapid accumulation of glycerol and glycerol 3 phosphatase becomes a more effective factor after glycerol fermentation reached into slowly accumulation phase. In a conclusion, it is a fundamental for C.glycerolgenesis over producing high level of glycerol that its cytoplasmic glycerol 3 phosphate dehydrogenase maintains at high activity and the activity of glycerol 3 phosphatase is much higher than that of cytoplasmic glycerol 3 phosphate dehydrogenase.
%K Glycerol
%K Candida glycerolgenesis
%K Mitochondrial glycerol-3-phosphate dehydrogenase
%K cytoplasmic glycerol 3-phosphate dehydrogenase
%K glycerol 3-phosphatase<br>甘油
%K 产甘油假丝酵母
%K 线粒体3-磷酸甘油脱氢酶
%K 胞浆3-磷酸甘油脱氢酶
%K 3-磷酸甘油酯酶
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=AB1D52041C5A8C28&yid=9806D0D4EAA9BED3&vid=1371F55DA51B6E64&iid=0B39A22176CE99FB&sid=F1A8654ADB4E656E&eid=3E0812ED84A7B31D&journal_id=0001-6209&journal_name=微生物学报&referenced_num=17&reference_num=29