%0 Journal Article
%T Cloning and Functional Analysis of the Candida glycerinogenes TRP1 Gene<br>产甘油假丝酵母TRP1基因的克隆与功能分析
%A SHEN Wei
%A WANG Zheng-Xiang
%A LI Yan-Li
%A WANG Dan
%A RAO Zhi-Ming
%A FANG Hui-Ying
%A ZHUGE Bin
%A ZHUGE Jian
%A <br>沈微
%A 王正祥
%A 李艳丽
%A 王丹
%A 饶志明
%A 方慧英
%A 诸葛斌
%A 诸葛健
%J 微生物学通报
%D 2009
%I 
%X Candida glycerinogenes WL2002-5 is an osmotolerant yeast used for the commercial production of glycerol. TRP1 gene of Candida glycerinogenes (CgTRP1), encoding phosphoribosyl anthranilate isomerase(PRA1) was cloned by complementation of the trp1 mutation of Saccharomyces cerevisiae W303-1A. Sequence analysis revealed the presence of a 735 bp open reading frame(ORF) encoding 244 amino acids protein, which shares 32.9 %~49.2% amino acids sequence similarity to PRAI proteins from other species of Saccharomycetales. Functional analysis reveal, high copy number of CgTRP1 can complement the trp1 mu-tation of S. cerevisiae completely, but low copy number of CgTRP1 can only partially complement the trp1 mutation. Downstream from CgTRP1 was found a gene highly similar to IPP1 gene which encoding inor-ganic pyrophosphatase. The nucleotides have been deposited in the GenBank database under accession No. EU078909.
%K Candida glycerinogenes
%K Phosphoribosyl anthranilate isomerase
%K trp1 mutation<br>产甘油假丝酵母
%K 磷酸核糖氨基苯甲酸同分异构酶
%K trp1缺陷
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=259015EB8DC5D8D2084194675770926F&yid=DE12191FBD62783C&vid=933658645952ED9F&iid=708DD6B15D2464E8&sid=82D2CD7E370B154A&eid=ABD3F52B9C9810A2&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=0