%0 Journal Article
%T Detection of CymMV in Orchids Tissue by Dot-ELISA and Tissue Blot-ELISA with Monoclonal Antibody<br>单抗免疫斑点法和组织印迹法检测侵染蝴蝶兰的建兰花叶病毒
%A DONG Xiao-Hui
%A MENG Chun-Mei
%A LI Jun-Ying
%A WU Jian-Xiang
%A RONG Song
%A ZHANG Chao
%A HONG Jian
%A <br>董晓辉
%A 孟春梅
%A 黎军英
%A 吴建祥
%A 荣 松
%A 张 超
%A 洪 健
%J 微生物学通报
%D 2009
%I 
%X Dot-ELISA and tissue blot-ELISA with monoclonal antibody to detect Cymbidium mosaic virus (CymMV) were established. 8000-dilution CymMV monoclonal antibody could be adopted to detect CymMV infecting leaf extract within a dilution limitation of 1:10240. CymMV could still be detected in the eighth print by tissue blot-ELISA . Similar results were shown between the fifth print by tissue blot-ELISA and 80-dilution of infected leaf extract by dot-ELISA. Comparison tests showed that the commonly used tissue blot-ELISA was less sensitive than dot-ELISA. It could illustrate that CymMV monoclonal antibody was more specific and sensitive than the polyclonal antibodies. The improved tissue blot-ELISA is more simple than dot-ELISA, and is suitable for fast detection with a large number of samples in the field.
%K Cymbidium mosaic virus
%K Monoclonal antibody
%K Dot-ELISA
%K Tissue blot-ELISA<br>建兰花叶病毒
%K 免疫斑点法
%K 组织印迹法
%K 单克隆抗体
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=BC87EE5A977CD587D0E8C6D6B78BB762&yid=DE12191FBD62783C&vid=933658645952ED9F&iid=F3090AE9B60B7ED1&sid=B0D0FAC45E96482A&eid=1A9481F9E49EB812&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=1&reference_num=0