%0 Journal Article
%T DETECTION OF PATHOGENIC EDWARSIELLA TARDA<br>致病性迟缓爱德华菌的检测
%A Xiong Qingming Lu Chengping
%A <br>熊清明
%A 陆承平
%J 微生物学报
%D 2001
%I 
%X 25 Edwardsiella tarda(Et) strains had been detected both on their viruslent factor Excellular Product (ECP), including the hemolysin and extracellular protease (ECPase), and on their pathogenicity to mice and Xiphophorus helleri. ECP was detected by Dot-ELISA with rabbit antiserum against ECP of reference strain JEL4. The results showed that the animal pathogenicity of Et had good correlation with its hemolysin other than with ECPase. The agreement between Dot-ELISA of JEL4 ECP and pathogenicity to animal was up to 100%. It was desirable to establish a detecting method, which only need detect the ECP with plate assay (PA) and Dot-ELISA, but needn't have animal experiment. Furthermore it is possible to develop a diagnosis kit of application to simplify the detecting procedure of pathogenic Et.
%K Edwardsiella tarda
%K Hemolysin
%K ECPase
%K Dot-ELISA<br>迟缓爱德华菌
%K 溶血素
%K 胞外蛋白酶
%K Dot-ELISA
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=093B65A5BC362672&yid=14E7EF987E4155E6&vid=2001E0D53B7B80EC&iid=B31275AF3241DB2D&sid=FA98B938D085B826&eid=3EE58D91F4253193&journal_id=0001-6209&journal_name=微生物学报&referenced_num=2&reference_num=24