%0 Journal Article
%T Study on L-cystine Conversion Technology by Pseudomonasputida TS1138<br>恶臭假单胞菌TS1138转化生产L-胱氨酸的工艺研究
%A LIU Chun-Qin
%A YU Yang-Sheng
%A BAI Gang
%A YANG Wen-Bo
%A CHEN Ning
%A HUAI Li-Hua
%A <br>刘春琴
%A 余养盛
%A 白 钢
%A 杨文博
%A 陈 宁
%A 怀立华
%J 微生物学通报
%D 2008
%I 
%X A technology of L-cystine production was studied in this paper, which included microbial enzymatic conversion of DL-2-amino-?2-thiazoline-4-carboxylic acid (DL-ATC) to L-cysteine, subsequent oxidization of L-cysteine to L-cystine and its purification. The cells of Pseudomonas putida TS1138 could be repetitively used as the enzyme sources to convert the substrate DL-ATC to L-cysteine. After being oxidated by 2% dimethy-sulforide (DMSO), L-cystine could be harvested and further purified by the positive ion-exchange resin 001×7. High Performance Liquid Chromatography (HPLC) identified the purified L-cystine as having a total recovery of 78.55% and purity of 99.12%. This study demonstrated an efficient and convenient method for L-cystine production, which overcame the instability of enzymes, troublesome procedures and high cost of enzyme immobilization as contrasted to the traditional method. All in all, it provides a new approach for industrial production of L-cystine as well as L-cysteine.
%K Pseudomonas putida
%K DL-ATC
%K L-cysteine
%K L-cystine
%K Production process<br>恶臭假单胞菌
%K DL-ATC
%K L-半胱氨酸
%K L-胱氨酸
%K 生产工艺
%K 恶臭假单胞菌
%K 转化
%K 生产
%K 胱氨酸
%K 工艺研究
%K putida
%K Pseudomonas
%K Technology
%K Conversion
%K 问题
%K 成本
%K 酶方法
%K 固定化
%K 使用
%K 酶稳定性
%K 总收率
%K 考察
%K 高效液相色谱法
%K 新工艺
%K 离子交换树脂纯化
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=7DD8DD4189CAFFD3D257F232B75F76B6&yid=67289AFF6305E306&vid=6209D9E8050195F5&iid=CA4FD0336C81A37A&sid=94E7F66E6C42FA23&eid=2A3781E88AB1776F&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=2&reference_num=15