%0 Journal Article
%T Effect of NaCl stimulation on the survival rate of freeze-dried cells and intracellular phosphofructokinase in Lactobacillus<br>NaCl刺激对乳杆菌冻干存活率及胞内磷酸果糖激酶的影响
%A ZHANG Zhong-Qing
%A LI Chun
%A LIU Ning
%A <br>张中青
%A 李春
%A 刘宁
%J 微生物学通报
%D 2011
%I 
%X NaCl stimulation affects the activity of phosphofructokinase (PFK) and survival rate of freeze-dried Lactobacillus bulgaricus. In the late logarithmic phase, adding 2% (W/V) NaCl to the growth medium markedly increased the survival rate of freeze-dried L. bulgaricus. Meanwhile, the activity of PFK in L. bulgaricus after NaCl stimulation was determined by reversed phase high performance liquid chromatography (RP-HPLC). The results showed that PFK activity was increased significantly by NaCl stimulation. In addition, a semi-quantitative RT-PCR method was applied to detect the PFK gene expression in different condition. The results indicated that the PFK gene expression was increased before freeze-drying and almost unchanged after freeze-drying. NaCl stimulation could enhance the survival rate of freeze-dried cells, and PFK might affect the survival of Lactobacillus bulgaricus.
%K Lactobacillus bulgaricus
%K Freeze-drying
%K NaCl stimulation
%K Phosphofructokinase
%K Semi-quantitative RT-PCR<br>保加利亚乳杆菌
%K 冷冻干燥
%K NaCl刺激
%K 磷酸果糖激酶
%K 半定量RT-PCR
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=D1920AB56F28E37C3987E882C0A01C0E&yid=9377ED8094509821&vid=16D8618C6164A3ED&iid=F3090AE9B60B7ED1&sid=EE882EEBF9236453&eid=0E6C9B38667ABC5C&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=13