%0 Journal Article
%T Protoplast Preparation and Regeneration of Monilia fructigena<br>桃褐腐病菌(Monilia fructigena)原生质体制备及再生条件
%A XUE Wei
%A ZHAO Xiao-Meng
%A LIU Su-Hu
%A ZHAO Xiao-Yan
%A LIU Zheng-Ping
%A <br>薛伟
%A 赵筱萌
%A 刘素花
%A 赵晓燕
%A 刘正坪
%J 微生物学通报
%D 2010
%I 
%X The condition of protoplast preparation and regeneration of Monilia fructigena was studied in this research. The results showed that the composition of cell wall degradative enzymes, liquid medium, mycelial age, digesting temperature and time duration affected the preparation of protoplast. More proto-plast of M. fructigena were yielded when mycelia incubated in Fries(1/2) for 24 h were digested by en-zyme mixture of 10 mg/mL driselase, 20 mg/mL snailase, 5 mg/mL celluase and 10 mg/mL lysozyme for 4 h at 28?C. The protoplast regeneration rate was affected by osmotic solution, solid regeneration medium, and digesting time.The best regeneration of protoplast of M. fructigena was achieved by using STC as isotonic solution and Fries(1/2) solid medium as regeneration medium. The regenerated strain of M. fruc-tigena was same as original strain in colony morphology and pathogenicity.
%K Monilia fructigena
%K Protoplast
%K Regeneration<br>桃褐腐病菌
%K 原生质体
%K 再生
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=0EF50F681323E86CB6A3C2A184342DC7&yid=140ECF96957D60B2&vid=42425781F0B1C26E&iid=CA4FD0336C81A37A&sid=F974714021A622E4&eid=5821AFD225844440&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=0