%0 Journal Article
%T Evaluation of Agar Disc Diffusion Method for Determining Antibiotic Susceptibility of Lactic Acid Bacteria Strains by a Selected Culture Medium
益生乳酸菌的纸片扩散法药敏性试验评价
%A FENG Da-Wei ZHOU Jia-Chun
%A
冯大伟
%A 周家春
%J 微生物学通报
%D 2010
%I
%X The evaluation of antibiotic susceptibility of lactic acid bacteria (LAB) is a major part of the safety of probiotic foods. However, there is no international standard method of the susceptibility testing of LAB till now. We investigated 6 culture media and selected a proper commercial one from them. We optimized the agar disc diffusion method and assayed 9 strains of LAB encompassing the genera Lactobacillus, Bifidobacterium, Streptococcus and Lactococcus for susceptibility to 51 antibiotics on RCA agar. The optimized method proved to be an applicable technique for antibiotic susceptibility testing of LAB. This contributes to the establishment of a standardized procedure for antibiotic susceptibility testing of LAB.
%K Probiotic
%K Lactic acid bacteria
%K Antibiotic susceptibility
%K RCA culture medium
%K Agar disc diffusion
益生菌
%K 乳酸菌
%K 药敏性
%K RCA培养基
%K 纸片扩散法
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=4CFF5498988BBB774E45B82B95BB5CB4&yid=140ECF96957D60B2&vid=42425781F0B1C26E&iid=38B194292C032A66&sid=4F772C43C525B07D&eid=33CD7A0E765CF51E&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=0