%0 Journal Article
%T Verification and Practical Application of BDV Nucleoprotein FQ RT-PCR Kit<br>BDV核蛋白FQ RT-PCR试剂盒的评价与应用
%A XU Ming-Ming
%A ZHAN Qun-Ling
%A ZHANG Ying-Ying
%A ZHANG Liang
%A SONG Wu-Qi ZHANG Feng-Ming XIE Peng
%A <br>徐鸣明
%A 展群岭
%A 张英英
%A 张亮
%A 宋武琦
%A 张凤鸣
%A 谢鹏
%J 微生物学通报
%D 2010
%I 
%X In order to verify the Fluorescent-Quantitation PCR kit specific for Borna disease virus (BDV) nucleoprotein, compare molecular beacon with common probe and study its practical application, the sensitivity, distinctness, repeatability and stability were tested on BDV and non-BDV infected cells, as well as non-infected controls. Human and animal samples were also tested by the kit. The lowest quantity of virus RNA detected by the kit was 2.5×101, which corresponded to 1 virus copy. No false positive result was found. The coefficient of variation (CV) of different batches was less than 0.7, and the CV between normal kits and the kits treated by accelerating decomposition was less than 2. The morbidities of human and animal detected by the kits were 3.6% (human), 4.2% (swine) and 1.5% (horse). The sensitivity and distinctness of this kit are better than common probe kit. All of the results indicate that this kit is an efficient tool in fundamental research, clinical detection and epidemiological investigation of BDV.
%K Borna Disease Virus
%K Real time PCR
%K Nucleoprotein<br>博尔纳病病毒
%K 实时荧光定量PCR
%K 核蛋白
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=4EF92EA5D6CAD79A0057907D6EC9B516&yid=140ECF96957D60B2&vid=42425781F0B1C26E&iid=0B39A22176CE99FB&sid=F66E8589980775DA&eid=24CDF81232C303C9&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=0