%0 Journal Article
%T Identification and Characterization of an Antagonistic Strain L1 Against Phyricularia grisea<br>水稻稻瘟病拮抗菌L1鉴定及抑菌特性的初步研究
%A LI Yong-Gang
%A SONG Xing-Shun
%A MA Feng-Ming
%A WEN Jing-Zhi
%A ZHAO Xue-Ying
%A <br>李永刚
%A 宋兴舜
%A 马凤鸣
%A 文景芝
%A 赵雪莹
%J 微生物学通报
%D 2008
%I 
%X On the basis of morphological, physiological and biochemical characterization, the antagonistic bacterium strain L1 against Phyricularia grisea was identified. The strain L1 was bacilliform, Gram-positive, fuscous and draped colonies, aerobiosis. The result showed that the strain L1 belongs to Bacillus subtilis. The antimicrobial activity of fermentation liquid reached the highest after 5 days-old cultures. And inhibitory rate accounted for 74.57%, but it had not changed along with the time increase. The activity effect of antimicrobial substances was insensitive to heat. The filtrated Bacillus subtilis strain L1 was precipitated in grades with the ammonium sulfate to draw the antimicrobial proteins and crude proteins were obtained. One crude protein drawn with ammonium sulfate at 50%~60% saturation showed strong antimicrobial activities to Phyricularia grisea. Average radius was accounted for 0.51 cm.
%K Biological control
%K Bacillus subtilis
%K Antimicrobial proteins
%K Identification<br>生物防治
%K 枯草芽孢杆菌
%K 拮抗蛋白
%K 鉴定
%K 水稻稻瘟病
%K 拮抗菌
%K 抑菌特性
%K 研究
%K Strain
%K Characterization
%K 半径
%K 抑菌效果
%K 粗蛋白质
%K 沉淀法
%K 饱和度
%K 硫酸铵梯度
%K 提取
%K 温度不敏感
%K 活性物质
%K 抑菌率
%K 抑菌活性
%K 发酵液
%K 培养
%K 枯草芽孢杆菌
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=6A299D71491AAEC67185C42C7F02AE01&yid=67289AFF6305E306&vid=6209D9E8050195F5&iid=B31275AF3241DB2D&sid=75B5F9C20EBE12E7&eid=1281D8F982952392&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=15