%0 Journal Article
%T Isolation, Identification and Characterization of Aeromonas hydrophila from Hemorrhagic Grass carp<br>草鱼出血病混合感染的嗜水气单胞菌的分离、鉴定与理化特性
%A DENG Guo-Cheng
%A JIANG Xiao-Yan
%A YE Xing
%A LIU Ming-Zhi
%A XU Shu-Ying
%A LIU Li-Hui
%A BAI Yue-Qiang
%A LUO Xia
%A <br>邓国成
%A 江小燕
%A 叶 星
%A 刘明智
%A 许淑英
%A 刘礼辉
%A 白岳强
%A 罗 霞
%J 微生物学通报
%D 2009
%I 
%X Two strains of infectious bacteria and a GCRV-like virus were isolated from grass carp which seemed to be infected by both virus and bacteria in Guangdong and Fujian provinces. CIK cells were inoculated with tissue fluid filtrated from the diseased fish. GCRV-like virus particles and virus inclusion body were observed in the cytoplasm of the infected cells under electron microscope. Artificial infection experiments showed that healthy grass carp died after infected with both the bacteria strains and virus-infected cell culture supernatant. Bacteria morphology, characteristics, physiology and biochemistry assays were used to identify the isolated bacteria. The characteristics of the two strains isolated were in accordance with those of Aeromonas hydrophila. PCR was performed to identify the bacteria. Both 16S rRNA gene and gryB gene of the two strains possessed high similarity with those of A. hydrophila registered in GenBank. AerA and ahpA genes could be amplified in both strains which indicated that both of them possessed strong pathogenicity. The two strains were therefore identified to be virulent strains of A. hydrophila. The diseased grass carp analyzed was supposed to be mixed infected by both A. hydrophila and virus.
%K Grass carp
%K Hemorrhage virus
%K Aeromonas hydrophila
%K Mixed infection
%K 16s rRNA
%K gyrB
%K aerA
%K ahp<br>草鱼
%K 出血病
%K 嗜水气单胞菌
%K 混合感染
%K 16S
%K 核糖体基因
%K 促旋酶B亚单位蛋白基因
%K 气溶素基因
%K 丝氨酸蛋白酶基因
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=799F0D8335AE630918A58487077EA3B0&yid=DE12191FBD62783C&vid=933658645952ED9F&iid=5D311CA918CA9A03&sid=75AB4EF959CC4ED9&eid=A60ED5C9472B8BEB&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=3&reference_num=23