%0 Journal Article
%T Establishment of Gel Electrophoresis for the Analysis of Peroxidases in Photosynthetic Bacteria Based on Genetic Algorithm<br>基于遗传算法的光合细菌过氧化物酶电泳分析方法的建立
%A NIU Hong-Jun LI Fei-Ying QIU Li-Xia YANG Guan-E
%A <br>牛红军
%A 李飞莹
%A 仇丽霞
%A 杨官娥
%J 微生物学通报
%D 2010
%I 
%X To establish the acid polyacrylamide gel electrophoresis (A-PAGE) analyzing method of peroxidase isoenzymes in photosynthetic bacteria. According to the number and the grey scale of bands, the conditions of the A-PAGE were optimized by the orthogonal design and the genetic algorithm. The results indicated that when the Triton X-100 concentration in the gels was 0.31%, the ultrasonic time was 19 min, the Triton X-100 concentration in the samples was 0.79%, the clear peroxidase isoenzyme zymograms of Rhodopseudomonas palustris were obtained, when the Triton X-100 concentration in the gels was 0.27%, the ultrasonic time was 16 min, the Triton X-100 concentration in the samples was 0.76%, the clear peroxidase isoenzyme zymograms of Rhodobacter sphaeroides were obtained. The paper set up a simple and rapid method for the analysis of peroxidase isoenzymes in photosynthetic bacteria.
%K Photosynthetic bacteria
%K Peroxidase isoenzymes
%K Polyacrylamide gel electrophoresis
%K Genetic algorithm<br>光合细菌
%K 过氧化物酶同工酶
%K 聚丙烯酰胺凝胶电泳
%K 遗传算法
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=89AEB4FB783E1AC1AC35E9398C4A1F3A&yid=140ECF96957D60B2&vid=42425781F0B1C26E&iid=38B194292C032A66&sid=87046BDD4E05C0FD&eid=63862ACCB0BCEB15&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=0