%0 Journal Article
%T Isolation of facultative anaerobic entophytic bacterial companioned Ca Las infected citrus tissues and evaluation of dominant bacterial populations<br>亚洲韧皮杆菌兼性厌氧型伴生细菌鉴定及优势菌群分析
%A LI Yan-Fang
%A YIN You-Ping
%A WANG Yu-Xi
%A LI Jia
%A CHEN Shi-Wei
%A WANG Zhong-Kang
%A <br>李颜方
%A 殷幼平
%A 王玉玺
%A 李佳
%A 陈世伟
%A 王中康
%J 微生物学通报
%D 2011
%I 
%X To analysis the entophytic bacterial diversity of citrus and find the companion bacteria populations associated with Huanglongbing pathogen-infected and healthy citrus plant tissues for decipher the co-cultivation of HLB pathogen, we selected varied parts of citrus tissues collected from different loca-tions of citrus planted area. The facultative anaerobic entophytic bacteria were isolated and purified based on bacterial morphology, physiology, biochemistry characteristics and the molecular method of PCR-DGGE (Denaturing gradient gel electrophoresis) analysis based on the sequence of 16S rRNA V6-V8 fragment gene. By the directional isolation of the facultative anaerobic entophytic bacteria and 16S rDNA amplification, total 12 genera of bacteria were identified from 19 cultivable bacterial popula-tions. The dominant bacterial population in infected citrus plants were Curtobacterium sp. (IF: 29.07%), Bacillus sp. (IF: 23.12%), Microbacterium sp. (IF: 21.09%) while in healthy citrus tissues belonged to Bacillus sp. (IF: 21.03%), Planococcus sp. (IF: 20.69%), Pseudomonas sp. (IF: 17.44%). From 50 target bands obtainded by the DGGE approach, 9 genera of cultivable bacteria were recognized. The dominant bacterium population belonged to Serratia sp. (IF: 28%) and Pantoea sp. (IF: 14%) followed by it. Can-didatus Liberibacter asiaticus was only found in tangerine pith of deformed orange fruit, which sug-gested that the content (>1%) of Huanglongbing was more in diseased fruits and other tissues of citrus had low abundance percentage. The density and species of entophytic bacteria were also observed in re-markable difference between infected and healthy citrus plant from the PCR-DGGE profiles.
%K Huanglongbing
%K Entophytic bacteria
%K Directional isolation
%K 16S rRNA diversity analysis<br>黄龙病
%K 内生细菌
%K 定向分离培养
%K 16S
%K rRNA多样性分析
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=D1920AB56F28E37CEEF787F6F8F4295E&yid=9377ED8094509821&vid=16D8618C6164A3ED&iid=9CF7A0430CBB2DFD&sid=6300C37F864D5DEA&eid=CDE3E49FCD9EE463&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=19