%0 Journal Article
%T Effects of Trace Elements on Growth and Acetate Production of Escherichia coli<br>微量元素对大肠杆菌生长和乙酸生成的影响研究
%A 朱才庆
%A 叶勤
%J 微生物学报
%D 2004
%I 
%X The metabolic characteristics of Escherichia coli DA19 were affected by the trace elements added in the minimal medium. When cultured in the minimal medium with an initial NH 4 + concentration of 17.4 mmol/L, both growth and cell yield based on consumed glucose were improved and less acetate was produced as a mixture of trace elements was added, and NH 4 + became a limiting nutrient at glucose concentration higher than 5.45g/L. When cultured in the minimal medium with sufficient nitrogen source (57.5 mmol/L of NH 4 +), addition of trace elements resulted in a great increase in cell growth and glucose consumption. The cell yield based on consumed glucose increased by at least 60% while produced acetate was only 13% of those obtained in medium without the addition of trace elements. The amount of added trace elements mixture, ranging from 0.1 to 1 mL/L, had almost the same effects on cell growth, acetate production and glucose consumption. Al 3+ had negative effects on the growth and glucose utilization while Co 2+ , Mn 2+ or Fe 2+ respectively improved growth and reduced acetate production. On the other hand, BO 3 3- , Zn 2+ , MoO 4 2+ , or Cu 2+ almost had no significant effects on the metabolic activities of DA19. In the medium supplemented with Fe 2+ , the behavior of the culture of DA19 was almost the same as that in medium supplemented with the trace element mixture.
%K Escherichia coli
%K Cell growth
%K Acetate
%K Trace elements<br>大肠杆菌，
%K 菌体生长，
%K 乙酸，
%K 微量元素
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=0196B2030FE90CFD&yid=D0E58B75BFD8E51C&vid=1AE5323881A5ECDC&iid=0B39A22176CE99FB&sid=D6354F61445E9456&eid=D9AE183D3F5C3C75&journal_id=0001-6209&journal_name=微生物学报&referenced_num=5&reference_num=25