%0 Journal Article
%T Isolation and Characterization of a New High Efficient H2-producing Bacterium Enterococcus sp. LG1<br>高效产氢菌株Enterococcus sp. LG1的分离及产氢特性
%A LI Yu-Liang
%A LI Xiao-Ming
%A GUO Liang
%A ZHOU Yi
%A ZENG Guang-Ming
%A YANG Qi
%A LIAO De-Xiang
%A <br>李宇亮
%A 李小明
%A 郭 亮
%A 周 屹
%A 曾光明
%A 杨 麒
%A 廖德祥
%J 微生物学通报
%D 2008
%I 
%X 12 strains of H2-producing bacteria were isolated and purified from anaerobic sludge, aerobic sludge and river bottom sludge by Hungate method. A new species of high efficient hydrogen production bacterium Enterococcus sp. LG1 (registration number: EU258743 ) was studied deeply. It was showed that the Enterococcus sp. LG1 was an anaerobic and Gram-negative bacterium. Sequence analysis of this type of clones showed that it was affiliated with the genus Enterococcus and it was not reported yet in other paper at present. Meanwhile, batch tests of anaerobic fermentative hydrogen production by Enterococcus sp. LG1 were investigated by using sterilization pretreated sludge as substrate. The changes of soluble COD, protein, carbohydrate and pH value during hydrogen fermentation were monitored. It was found that only hydrogen and carbon dioxide were produced by this strain and no methane was detected during fermentation. The maximal hydrogen yield was 36.48 mL/g TCOD and the hydrogen concentration in the gas phase was 73.5%. The Enterococcus sp. LG1 was a butyrate fermentation bacteria analyzed by metabolites.
%K Bio-hydrogen
%K Novel species
%K Sludge
%K Anaerobic fermentation
%K Hydrogen producing function<br>生物产氢
%K 新菌
%K 污泥
%K 厌氧发酵
%K 产氢能力
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=737912381302C6B2B924491AD7DA3A73&yid=67289AFF6305E306&vid=6209D9E8050195F5&iid=9CF7A0430CBB2DFD&sid=A126866E01788114&eid=9129323FE7AA9847&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=14