%0 Journal Article
%T Rapid Extraction of Filamentous Fungal DNA for PCR Amplification<br>一种用于PCR扩增的丝状真菌DNA快速提取方法
%A PAN Li
%A CUI Cui
%A WANG Bin
%A <br>潘力
%A 崔翠
%A 王斌
%J 微生物学通报
%D 2010
%I 
%X Filamentous fungi play an important role in industry, agriculture, medicine and biological fundamental research. More and more attention has been paid to fungal breeding and gene functional analysis via genetic transformation technology. However, the extraction of fungal DNA was labor intensive and time-consuming, which makes it difficult to perform high-throughput screening of transformants through PCR technology. In this paper, a rapid DNA extraction method was established for filamentous fungi (take Aspergillus for example). DNA was easily obtained by exposing the fungal mycelia in 10 × TE buffer to microwave. RAPD and PCR experiments demonstrated that the extracted DNA satisfied the requirements of PCR amplification. These results provided a foundation for the high-throughput screening of filamentous fungal transformants.
%K Filamentous fungi
%K DNA extraction
%K Screening and identification
%K Transformant
%K RAPD<br>丝状真菌
%K DNA提取
%K 筛选鉴定
%K 转化子
%K RAPD
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=0831B74AC8EABFEA83DFF147633F15E3&yid=140ECF96957D60B2&vid=42425781F0B1C26E&iid=38B194292C032A66&sid=A4E991BEB6B99C46&eid=50977187B552C371&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=0