%0 Journal Article
%T Fermentative Hydrogen Production with Xylose by Clostridium sp. HR-1 Isolated from Cow Dung Compost<br>木糖发酵产氢菌的筛选及其生长产氢特性研究
%A XU Ji-Fei
%A REN Nan-Qi
%A QIU Jie
%A SU Dong-Xia
%A <br>许继飞
%A 任南琪
%A 邱 颉
%A 苏东霞
%J 微生物学通报
%D 2009
%I 
%X A anaerobic hydrogen-producing strain HR-1 was isolated from compost. Phylogenetic analysis based on 16S rRNA sequence similarity indicates that strain HR-1 is the closest relative to Clostridium acetobutylicum ATCC 824, with the similarity of 96%. Biological characteristics and phylogenetic analysis of 16S rRNA gene indicate that HR-1 is a new species named Clostridium sp. HR-1. Cells are Gram-positive, mobile rod-shaped. Spores and flagellums were no observed. Temperature range for growth is 10°C to 45°C (optimum temperature 37°C~39°C), and range pH for growth is 4.0 to 10.0 (optimum pH 7.5~8.0). H2, CO2, acetate, butyrate and a little ethanol are the end products of PYG fermentation. Strain HR-1 has the ability to use organic nitrogen and inorganic nitrogen sources for growth and hydrogen production, and yeast extract is the optimum nitrogen source for hydrogen production. Strain HR-1 produces hydrogen from xylose (3 g/L) at 37°C and initial pH 6.5, the hydrogen yields and maximal hydrogen production rate are 1.84 mol-H2/mol-xylose and 10.52 mmol-H2/h·g-cdw, respectively. Strain HR-1 is able to utilize glucose, galactose, fructose, mannose and cellobiose for hydrogen production and the hydrogen yields from glucose is 2.36 mol-H2/mol-glucose.
%K Fermentative hydrogen production
%K Isolation and characterization
%K Clostridium sp
%K HR-1
%K Xylose<br>发酵产氢
%K 分离与鉴定
%K Clostridium
%K sp.
%K HR-1
%K 木糖
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=2D1FE99BE7F7250B140ED5C4D4C5B68B&yid=DE12191FBD62783C&vid=933658645952ED9F&iid=E158A972A605785F&sid=9BAF8BADA5DAC376&eid=FC54572CA87B683E&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=1&reference_num=10