%0 Journal Article
%T Studies on Shedding Virus and the Formation of Aerosol of H9N2 Subtype AIV<br>H9N2亚型AIV排毒及形成气溶胶的研究
%A CAO Yong-Zhi
%A MA Wei-Ming
%A LI Xiao-Xi
%A CHAI Tong-Jie
%A ZHANG Hai-Yan
%A <br>曹永芝
%A 马卫明
%A 李晓霞
%A 柴同杰
%A 张海燕
%J 微生物学通报
%D 2008
%I 
%X In order to study the regularity of shedding virus from infected SPF chickens and the formation of aerosol of H9N2 subtype AIV, SPF chickens were bred in a positive and negative pressure isolator. Aerosol samples were collected by AGI-30 (All Glass Impinger-30) extractor, and simultaneously trachea and cloaca samples were collected by tracheal swabs and cloacal swabs in different periods after challenged with viruses. The above-mentioned samples were detected by HI, Dot-ELISA and RT-PCR methods. The results indicated that aerosols were isolated from the 4 days to the 43 days after inoculation. It was proved that H9N2 subtype AIV could copy themselves in respiratory passage and cloaca, and then could formation of aerosols. AIV H9N2 subtype could be isolated from cloacal and tracheal swabs 3 days after inoculation and lasted for 45 days, viruses were detected from all infected SPF chickens on 7 days.
%K Avian influenza
%K Shedding virus
%K Formation
%K Aerosol<br>禽流感
%K 排毒规律
%K 形成
%K 气溶胶
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=525CBB49E71D1776A1D8F86F97C69AB9&yid=67289AFF6305E306&vid=6209D9E8050195F5&iid=F3090AE9B60B7ED1&sid=371466E036DA0FD9&eid=633354CC2908E635&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=18