%0 Journal Article
%T Purification and N-terminal Amino Acid Sequencing of the ESM Protease Isolated from an Eggshell Membrane-degrading Bacteria<br>蛋壳内膜分解菌所产蛋白酶的纯化及其N-末端氨基酸序列测定
%A LI Bo
%A DANG Yong
%A MA Yu
%A CHEN Ying-Yi
%A <br>李 勃
%A 党 永
%A 马 瑜
%A 陈颖怡
%J 微生物学通报
%D 2008
%I 
%X A strain producing eggshell membrane protease (ESM protease) was isolated from the soil and identified as Pseudomonas aeruginosa. The enzyme isolated from the fermentation liquid of this strain and purified by ammonium sulfate precipitation, quadratic anion-exchange chromatography exhibited eggshell membrane degrading activity of 304.5 U/mg. By SDS-PAGE, the protein molecular mass is 32 kD. The N-terminal amino acid sequence of this protease is: Ala, Glu, Ala, Gly, Gly, Val, Ala, Gly, Lys, Glu, Asp, Ala, Ala, Glu, Leu.
%K ESM protease
%K Pseudomonas aeruginosa
%K Purification
%K N-termial amino acid sequencing<br>蛋壳内膜蛋白酶
%K 铜绿假单胞菌
%K 纯化
%K N-末端氨基酸测序
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=12E4C71A6FE64662469455B02379BD5E&yid=67289AFF6305E306&vid=6209D9E8050195F5&iid=5D311CA918CA9A03&sid=7CCBDF94263DBB99&eid=38FD6970749494EF&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=9