%0 Journal Article
%T Screening and Identification of a Cold-adapted Cellulase-producing Bacteria<br>一株耐低温纤维素酶高产菌株的筛选、鉴定和产酶的初步试验
%A HUANG Yu-Lan
%A LI Zheng
%A LIU Xiao-Ning
%A WANG Pei-Pei
%A ZHANG Jie
%A <br>黄玉兰
%A 李征
%A 刘晓宁
%A 王培培
%A 张杰
%J 微生物学通报
%D 2010
%I 
%X Strain XW-1 producing extracellular cellulose was isolated from 80 cm depth under the surface layer at Zoige Plateau Alpine Wetland. It was identified as a Brevundimonas sp. by its morphological, the physiological and biochemical properties and 16S rDNA sequence analysis. The results of cellulose producing research showed that the optimum carbon sourse was 0.5% CMC-Na, the optimum temperature was 20°C. Under this condition, the highest cellulose activity reached to 15.6 U/mL in the third day. Enzymatic properties showed that the optimum pH value is 6.0 and the optimum reaction temperature is 20°C, the relative enzyme activity reach to 80% at 15°C, moreover, at 5°C the relative enzyme activity still reach to 56%.
%K Zoige Alpine Wetland
%K Low cellulose
%K Brevundimonas
%K Enzymatic properties<br>若尔盖高寒湿地
%K 低温纤维素酶
%K 短波单胞菌
%K 酶学性质
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=618FCF31CBBAAC194174931999C7EDB2&yid=140ECF96957D60B2&vid=42425781F0B1C26E&iid=94C357A881DFC066&sid=99F56932E7A3212B&eid=C2B44FF23940A30A&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=0