%0 Journal Article
%T Screening, Identifying and Function Analysis of Polyketide Synthase I Cluster from the Environmental Strain X-2 Which Produce Macrolactins<br>产Macrolactins的海洋细菌X-2中Ⅰ型PKS基因簇的筛选鉴定与功能分析
%A DONG Xiao-Yi
%A WANG Liang-Hu
%A SUN Ming-Juan
%A ZONG Ying
%A JIAO Yu-Liang
%A JIAO Bing-Hua
%A <br>董晓毅
%A 王梁华
%A 孙铭娟
%A 宗 英
%A 焦豫良
%A 焦炳华
%J 微生物学通报
%D 2008
%I 
%X Macrolactins are 24-membered macrolides produced by unidentified marine bacterium, Actinomadura sp. and Bacillus sp., which exhibit both antibacterial and antitumor activities in vitro. The environmental strain X-2 which was isolated from the sediment of the East China Sea produce Macrolatin A, B and O. In this study, a set of degenerate oligonucleotide primers, designed for amplification ketosynthase(KS) domains, had been employed to identify KS gene fragments of the X-2 DNA samples. One 645 bp KS fragment(GenBank accession no. EF486351)had been cloned and used as a probe to screen the genome DNA fosmid library of X-2. Three positive clones were selected and sequenced, Homologous analysis and the function prediction of the obtained PKS gene fragments suggested that macrolactin is the Polyketide Biosynthesis Product of the gene cluster obtained in the environmental strain X-2.
%K Macrolactin<br>聚酮合酶
%K 枯草芽孢杆菌
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=7C733FD6A3A07959747C166A7E924F10&yid=67289AFF6305E306&vid=6209D9E8050195F5&iid=9CF7A0430CBB2DFD&sid=7264799D7F648D02&eid=40295B54D40AE4B3&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=1&reference_num=11