%0 Journal Article
%T Time-space Change of Methanogen Amount from Zoige Plateau<br>若尔盖高原产甲烷菌数量的时空差异性
%A TANG Bo
%A TANG Jie
%A WU Li-Sh
%A YANG Zhi-Rong
%A ZHANG Jie
%A <br>汤博
%A 唐杰
%A 吴俐莎
%A 杨志荣
%A 张杰
%J 微生物学通报
%D 2010
%I 
%X To quantify the population size of total methanogens changing with environments, season and depths of Zoige plateau, the plasmids and standard curves of species were constructed by using real - time absolute quantitative PCR. The concentration of the standard plasmids was 160 mg/L, with the coefficient being 0.992, and the amplification efficiency was 98.6%. The results showed that in April the population size of total methanogens in wetland ecosystem and grassland ecosystem were nearly the same. In wetland ecosystem, the population size of total methanogens decreased in the order of September, July, and April, while increased with the sampling depth in September and July, In grassland ecosystem, however, the population size of total methanogens did not change significantly with season and depth.
%K Zoige plateau
%K Real-time PCR
%K Methanogens<br>若尔盖高原
%K 实时荧光定量PCR
%K 甲烷菌
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=21123E9AEF0C6B4AA5F226DDCD0123C4&yid=140ECF96957D60B2&vid=42425781F0B1C26E&iid=708DD6B15D2464E8&sid=0CCE46DAE11318DE&eid=A22DD5EE0F220B37&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=0