%0 Journal Article
%T Isolation and Identification of Strain ZY-23 Producing Tetrodotoxin<br>产河豚毒素(TTX)菌株ZY-23的分离与鉴定
%A CHI Zhen
%A ZHENG Ying
%A MAO Ning
%A <br>池珍
%A 郑莺
%A 毛宁
%J 微生物学通报
%D 2010
%I 
%X A total of 35 bacterial strains were isolated from the ovarian of Globefish. It was confirmed that 11 bacterial strains among them were capable of producing tetrodotoxin using mouse bioassay method and the toxin concentrations in the cultures of 6 bacterial strains were above 400 ng/mL. Based on the morphology, physiological and biochemical characteristics, the strain ZY-23, one of the bacterial strains, was identified preliminarily as Serratia liquefaciens. The phylogenetic analysis showed that 16S rRNA sequence of the bacterial strain ZY-23 had 99% homology to that of Serratia sp. Tp5. The result of fluorimetric spectrophotometry indicated that there was the same fluorescence peak of the fermentation broth as that of the standard substance, suggesting that the strain ZY-23 indeed produced tetrodotoxin.
%K Tetrodotoxin
%K Mouse bioassay method
%K Physiological and biochemical research
%K 16S rRNA
%K Serratia liquefaciens
%K Fluorescent spectrometry<br>河豚毒素
%K 小鼠生物检测法
%K 生理生化试验
%K 16S
%K rRNA
%K 液化沙雷氏菌
%K 荧光检测法
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=32E4A30EE1DB70B36A90C716637DD9EC&yid=140ECF96957D60B2&vid=42425781F0B1C26E&iid=0B39A22176CE99FB&sid=B5A64C4F3BFD067A&eid=E11C591EBA070644&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=1&reference_num=0