%0 Journal Article
%T Rapid species identification of Mycobacterium tuberculosis by rpoB Gene Micro Array<br>利用rpoB基因芯片技术进行快速分枝杆菌菌种鉴定
%A LI Hong-Min
%A FAN Bo
%A WANG Wei
%A LI Guo-Li
%A MIAO Qing
%A <br>李洪敏
%A 樊 博
%A 王 巍
%A 李国利
%A 苗 青
%J 微生物学通报
%D 2008
%I 
%X Rapid species identification of Mycobacterium tuberculosis by rpoB gene micro array. Based on the gene micro array of rpoB, the standard strains of 21 mycobacteria and 8 non-mycobacteria ,126 clinical isolated of mycobacteria were detected by PCR-reverse dot blot hybridization assay. 360bp DNA fragment was amplified from all mycobacteria tested and was not found in all nonmycobacteria except hemolytic streptococcus and corynebacterium pseudodiphtheriticum. The result of specimens were detected by the probe which is composed of 21 oligonucleotide was that probe-Mycobacterium fortuitum cross hybridizated with Mycobacterium platypoecilus while the other probes were specific. The 89 strains of the all 126 strains isolated from clinical specimens were identified to be mycobacterium tuberculosis, the percentage was 70.6%(89/126), while the other 9 strains were identified to be unmycobacterium tuberculosis and the the percentage was 9.2%(9/98). Identification of Mycobacteria by rpoB gene micro array is a rapid and effective method which is of considerable value in clinical territory.
%K Mycobacterium
%K rpoB gene micro array
%K oligonucleotide probe
%K Nucleicacid hybridization<br>分枝杆菌
%K rpoB基因芯片
%K 寡核苷酸探针
%K 核酸杂交
%K 利用
%K rpoB
%K 基因芯片
%K 芯片技术
%K 快速
%K 非结核分枝杆菌
%K 菌种鉴定
%K Array
%K Micro
%K Gene
%K Mycobacterium
%K tuberculosis
%K Species
%K Identification
%K 价值
%K 临床应用
%K 方法
%K 技术鉴定
%K 杂交
%K 寡核苷酸探针
%K 海分枝杆菌
%K 样片
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=6C7BC559951B6BA54C1C499EC96F5719&yid=67289AFF6305E306&vid=6209D9E8050195F5&iid=CA4FD0336C81A37A&sid=A04140E723CB732E&eid=B91E8C6D6FE990DB&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=10