%0 Journal Article
%T ESTABLISHMENT OF TRANSFORMATION SYSTEM OF STREPTOMYCES LINCOLNENSIS<br>林可链霉菌转化系统的建立
%A Zhang Huizhan
%A Yao Feng
%A Ye Jiang
%A <br>张惠展
%A 姚峰
%A 叶江
%J 微生物学报
%D 1999
%I 
%X Transformation system of S. lincolnensis was established to improve lincomycin--producing strains by Gene retransformation Technology. Under optimal conditions, 10(11) protoplasts were prepared from 25 ml SM culture medium, and the regeneration frequency of protoplasts was 16%. The transformation frequency of pIJ702 was up to 5.5 x 10(5)/microgram DNA. The results revealed that gene manipulation in S. lincolnensis was facile as that in S. lividans.
%K Streptomyces lincolnensis
%K Protoplast regeneration
%K Transformation<br>林可链霉菌，原生质体再生，转化
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=89FA861EBABDADFAD319A69F4D1EA83E&yid=B914830F5B1D1078&vid=7C3A4C1EE6A45749&iid=CA4FD0336C81A37A&sid=E203FB1A272C9DD2&eid=06EA2770E96C5402&journal_id=0001-6209&journal_name=微生物学报&referenced_num=2&reference_num=1