%0 Journal Article
%T Use of Fluoresceinated Recombinant Phage to Study the Receptor/Ligand Interaction<br>利用荧光标记的T7噬菌体研究配体/受体的相互作用
%A LI Jiao
%A WANG Ai-Ping
%A ZHANG Hong
%A ZHANG Gai-Ping
%A WANG Xuan-Nian
%A YI Ming-Lin
%A LU Kun
%A WANG Qiu-Xia
%A FENG Li-Li
%A <br>李 姣
%A 王爱萍
%A 张 红
%A 张改平
%A 王选年
%A 易明林
%A 鲁 琨
%A 王秋霞
%A 冯丽丽
%J 微生物学通报
%D 2008
%I 
%X VP2 protein of infectious bursal disease virus(IBDV) was displayed on T7 phage surface, and this recombinant phage was then purified and labeled with FITC. The interaction between fluorescigenic phage and IBDV host cells was detected by fluorescent microscope and flow cytometer(FCM). Data shows that after displayed on labeled T7 phage suface, VP2 protein still remains its binding activities with IBDV host cells, and this interaction can be blocked by IBDV vaccine strain TAD in a does dependent manner, while no interaction was observed in negative control. The described method should find widespread application in the rapid in vivo research of interactions between ligands and receptors.
%K Phage display
%K FITC labeling
%K Infectious bursa disease virus
%K VP2 protein<br>噬菌体展示
%K FITC标记
%K 传染性法氏囊病病毒(IBDV)
%K VP2蛋白
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=CB9E37E9BC938A8EED3CC2289FC00112&yid=67289AFF6305E306&vid=6209D9E8050195F5&iid=5D311CA918CA9A03&sid=65C780F1B91D7CD5&eid=59D5B0EDB2D8DABC&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=1&reference_num=9