%0 Journal Article
%T Multiple Gene Inactivation Approach in Escherichia coli Mediated by a Combination of Red Recombination and Xer Recombination<br>基于Red重组系统和Xer重组系统的大肠杆菌多基因删除方法
%A ZHOU Li
%A NIU Dan-Dan
%A LI Ning
%A CHEN Xian-Zhong
%A SHI Gui-Yang
%A WANG Zheng-Xiang
%A <br>周丽
%A 牛丹丹
%A 李宁
%A 陈献忠
%A 石贵阳
%A 王正祥
%J 微生物学通报
%D 2010
%I 
%X Rapid and efficient inactivation of the target gene on Escherichia coli chromosome is the precondition and groundwork of researches on metabolic engineering. In the present study, we demonstrated a multiple gene inactivation approach in E. coli mediated by Red recombination and Xer recombination. The chromosomal genes, ackA-pta and pps, in a wild type strain E. coli CICIM B0013 were inactivated by this method indicating that dif sites can be reused to inactivate multiple chromosomal genes with no antibiotic resistance selectable marker remain. Furthermore, this method has high recombination efficiency and simplified steps.
%K Xer recombination
%K dif sites
%K Gene inactivation<br>Xer重组系统
%K dif位点
%K 基因删除
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=31ECAE7BCB47845BFEDF4C68FA1EF07D&yid=140ECF96957D60B2&vid=42425781F0B1C26E&iid=B31275AF3241DB2D&sid=EA60CDEE036FF0DF&eid=9DC3B794038556CA&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=0