%0 Journal Article
%T THE DETECTION OF PHENOL DEGRADING STRAIN IN ENVIRONMENT WITH SPECIFIC PRIMER OF PHENOL HYDROXYLASE GENE<br>采用苯酚羟化酶基因特异引物检测苯酚降解菌
%A Y Xu
%A X Fang
%A M Chen
%A W Zhang
%A J Li
%A M Lin
%A <br>徐玉泉
%A 方宣钧
%A 陈明
%A 张维
%A 李浚明
%A 林敏
%J 微生物学报
%D 2001
%I 
%X A 684 bp oligonucleotide fragment was produced by PCR amplification from phenol-degrading strain Acinetobacter calcoaceticus PHEA-2 with the specific primers of gene encoding phenol hydroxylase. The nucleotide sequence of this fragment and its deduced amino acid sequence share 84% and 98% homology with the phenol hydroxylase gene and its deduced amino acid sequences of phenol-degrading strain Acinetobacter calcoaceticus NCIB8250. Sets of different aromatic compounds degrading strains were used to test this specific prime. The 684 bp-fragments were amplified only from phenol-degrading strains by PCR. When using this pair of primers to detect the bacterial isolates from wastewater discharged from coking plant, all the tested strains, which possess 684 bp characteristic fragment, showed the ability to degrade phenol in this study.
%K Phenol\|degrading strains
%K Phenol Hydroxylase gene
%K PCR detection
%K Coking wastewater<br>苯酚降解菌
%K 苯酚羟化酶基因
%K PCR检测
%K 炼焦废水
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=9350697D17839366&yid=14E7EF987E4155E6&vid=2001E0D53B7B80EC&iid=38B194292C032A66&sid=88D36036CFF69B3C&eid=358F98408588E522&journal_id=0001-6209&journal_name=微生物学报&referenced_num=14&reference_num=17