%0 Journal Article
%T Improving of the Thermostability of Penicillium expansum Lipase by Creating Mutation P197E<br>P197E与ep8叠加突变对扩展青霉脂肪酶热稳定性的影响
%A LIU Yan-Y
%A HUANG Ping
%A SI Shen-Qian
%A CHEN Yan
%A LIN Lin
%A <br>刘燕雅
%A 黄平
%A 司圣乾
%A 陈彦
%A 林琳
%J 微生物学通报
%D 2010
%I 
%X In order to improve the thermostability of Penicillium expansum lipase (PEL), a mutation P197E was created by site-directed mutagenesis. The mutation is generated by overlap extension PCR using the cDNA of a single site mutant lipase ep8 as the template and two special primers that corresponding to mutation P197E. Recombinant vector pPIC3.5K-ep8-P197E which contain double-mutant genes was constructed and electroporated into Pichia pasteris GS115. The recombinant transformant was selected and grow in the methanol containing media for expressing double-mutant lipase, PEL-ep8-P197E. Thermostability analysis reveals that the residual activity of the double-mutant are 42.13% and 37.3% greater than that of the wild type PEL and PEL-ep8 after incubated at 40°C for 30 min. The Tm of the double-mutant lipase is 41.51°C, 2.81°C higher than PEL and 2.25°C higher than PEL-ep8.
%K Penicillum expansum lipase
%K Mutagenesis
%K Thermostability<br>扩展青霉脂肪酶
%K 叠加突变
%K 热稳定性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=F258B24947C95B731CA3E39F6C1D4177&yid=140ECF96957D60B2&vid=42425781F0B1C26E&iid=0B39A22176CE99FB&sid=BFFEB343E58C3A59&eid=D6C59076834E314A&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=0