%0 Journal Article
%T Isolation of a Rhodotourula mucilaginosa strain capable of transforming quinuclidinone into (R)-3-quinuclidinol and optimization of the transforming reaction conditions<br>转化奎宁酮为(R)-3-奎宁醇菌株的筛选及转化条件优化
%A QIU Jian
%A ZHANG Xi
%A MA Hong
%A ZHANG Duo
%A SONG Shui-Shan
%A <br>邱健
%A 张霞
%A 马宏
%A 张铎
%A 宋水山
%J 微生物学通报
%D 2011
%I 
%X (R)-3-Quinuclidinol is an important chiral building block for the synthesis of a variety of pharmaceutical. A strain which is able to transforming quinuclidinone into reductase (R)-3-Quinuclid inol was isolated from soil by using quinuclidinone as sole carbon sourse in the screening medium. The physiological and bio-chemical identification and 18S DNA sequense analysis revealed that this strain belongs to Rhodotorula mucilaginosa and was named as R. mucilaginosa X15. The present results indicated that the optimal conditions for the transforming reaction with resting cells of R. mucilaginosa X15 were 3-quinuclidinone 10 g/L, pH 7.5, 30 °C and 72 h reaction time. (R)-3-Quinuclidinol is obtained with an yeild of 90% and with an optical purity of 88% enantiometric excess (ee) from a 100 mL reaction mixture.
%K 3-Quinuclidinone reductase
%K Rhodotorula mucilaginosa
%K Asymmetric reduction
%K (R)-3-Quinuclidinol<br>奎宁酮酮基还原酶
%K 粘红酵母
%K 对称还原
%K (R)-3-奎宁醇
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=2C6838B4E216843611075BF90CA55399&yid=9377ED8094509821&vid=16D8618C6164A3ED&iid=94C357A881DFC066&sid=FA98B938D085B826&eid=81D76BB45305F8B7&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=11