%0 Journal Article
%T Cloning and Sequence Analysis of Dienelactone Hydrolase Gene<br>双烯内酯水解酶基因的克隆和序列分析*
%A ZHONG Wen-Hui SUN Ming HE Guo-Qing FENG Xiao-Shan YU Zi-Niu
%A <br>钟文辉
%A 孙明
%A 何国庆
%A 冯孝善
%A 喻子牛
%J 微生物学通报
%D 2004
%I 
%X A 2,4 -dichlorophenol degrading Pseudomonas strain GI241-1 was isolated from a soil sample. The dienelactone hydrolase gene, designated as dcpD which encodes dienelactone hydrolase involved in transforming cis-2-chloro-dienelactone into 2-chloromaleylacetic acid, was cloned from this bacterium strain. The gene cloning strategy was to construct genomic library after location of its neighbouring gene by Southem blot and to screen the aim transformant by dot blotting. Sequencing results showed that length of dcpD is 702bp. The sequence of dcpD and the deduced amino acid are different from the relative sequences registered in the GenBank.
%K Pseudomonas
%K 2
%K 4-Dichlorophenol
%K Dienelactone hydrolase gene
%K Gene cloning<br>假单胞菌
%K 2，4-二氯酚
%K 双烯内酯水解酶基因
%K 基因克隆
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=3EAB323A4E55147C&yid=D0E58B75BFD8E51C&vid=4AD960B5AD2D111A&iid=38B194292C032A66&sid=9C65ADEB5990B252&eid=656F8C8401D91023&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=13